Composite

Part:BBa_K3096045:Design

Designed by: Patrick Mueller, Lina Widerspick, Jakob Keck, Famke Baeuerle   Group: iGEM19_Tuebingen   (2019-10-15)


Tet prom. with Exendin-4 with a N-terminal secretion tag and a C-terminal cell penetrating peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 312
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Repeats in the TetR promoter region can cause trouble when deciding to synthesize this construct.


Source

Combination of previously designed parts.

References